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anti βactin  (Proteintech)


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    Structured Review

    Proteintech anti βactin
    Anti βactin, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 8212 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+%CE%B2actin/beta+Actin+Monoclonal+antibody/10__1080_slash_0886022x__2026__2736906-66-43-46
    Average 97 stars, based on 8212 article reviews
    anti βactin - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: GALNT1 emerges as a potential therapeutic target in breast cancer.
    Article Snippet: .. After blocking with 5% non-fat milk, the membranes were incubated overnight at 4 °C with primary antibodies, including anti-GALNT1 (1:1000, 21139-1-AP, Proteintech), anti-βactin (1:40000, 20536-1-AP, Proteintech), or anti-GAPDH (1:40000, 10494-1-AP, Proteintech). .. HRP-conjugated goat anti-rabbit IgG (1:5000, SA00001-2, Proteintech) was applied for 1 h at room temperature.

    Incubation:

    Article Title: GALNT1 emerges as a potential therapeutic target in breast cancer.
    Article Snippet: .. After blocking with 5% non-fat milk, the membranes were incubated overnight at 4 °C with primary antibodies, including anti-GALNT1 (1:1000, 21139-1-AP, Proteintech), anti-βactin (1:40000, 20536-1-AP, Proteintech), or anti-GAPDH (1:40000, 10494-1-AP, Proteintech). .. HRP-conjugated goat anti-rabbit IgG (1:5000, SA00001-2, Proteintech) was applied for 1 h at room temperature.

    Article Title: SOD1 lactylation impair its enzymatic activity by conformational change to aggravate intervertebral disc degeneration.
    Article Snippet: Proteins were resuspended in SDS– PAGE loading buffer (P0015, Beyotime), boiled, and separated by 10% or 12% SDS–PAGE, then transferred to PVDF membranes (Merck, Germany). .. Membranes were blocked with QuickBlock Western solution (P0252, Beyotime) and incubated overnight at 4 °C with primary antibodies, including anti-P21 (ab109199, Abcam), anti-GADD45α (ab54210, Abcam), anti-SOD1 (ab13498, Abcam), anti-pan lactylation (PTM-1401RM, PTM), anti-SOD1K123la (customized, PTM), anti-pan acetylation (PTM-101, PTM), anti-pan succinylation (PTM-419, PTM), and anti-βactin (66009-1-Ig, Proteintech) antibodies. .. After being washed with TBST, the membranes were incubated with secondary antibodies (SA00001-2 or SA00001-1, Proteintech).

    Article Title: Endogenous Ceramide 24:1 Constrains Th17-Driven Neutrophilic Inflammation by Antagonizing EP2 Signaling.
    Article Snippet: Advanced Science, 2026 om m ons L icense nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.202520695 by IN A SP - N E PA L , W iley O nline L ibrary on [15/03/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C For WB analysis, the protein lysates were separated via SDS ‒PAGE, transferred to PVDF membranes, and incubated overnight at 4◦C with the following primary antibodies: antiSmpd1 (1:1000, ABclonal, #39147-1), anti-Cers2 (1:1000, Abcam, #ab31545), anti-Sptlc2 (1:1000, Abcam, #ab229330), anti-Ror γt (1:1000, Proteintech, #29910-1-AP), anti-Jak2 (1:1000, CST, #3230), anti-p-Jak2 (1:1000, CST, #8082S), anti-Stat3 (1:1000, CST, #4904T), anti-p-Stat3 (1:1000, CST, #9145T), anti-EP2 (1:1000, Abcam, #ab167171), anti-EP4 (1:1000, Proteintech, #24895-1-AP), anti-CXCR4 (1:1000, Abcam, #ab181020), anti-CREB (1:1000, CST, #9197), anti-p-CREB (1:1000, CST, #9198), and anti- βActin (1:1000, Proteintech, #20536-1-AP). ..

    Article Title: METTL1-Mediated m7G Modification of GINS2 mRNA Activates STAT3 to Drive Esophageal Squamous Cell Carcinoma Progression.
    Article Snippet: Background: METTL1mediated m7G modification plays a procancer role in various malignant tumors, but whether it promotes the progression of esophageal squamous cell carcinoma (ESCC) by regulating the DNA replication factor GINS2 and tumorassociated macrophage polarization remains unclear.. Methods: The expression and function of GINS2 were examined through in vitro and in vivo experiments.. RIP, MeRIP, and RNA stability assays were used to validate the regulatory effect of METTL1 on GINS2 modification.

    Article Title: Programmable RNA N 6 ,2´-O-Dimethyladenosine Editing.
    Article Snippet: .. Followed by incubation with anti-GAPDH (Proteintech 60004-1-Ig, 1:100 000), anti-BICD2 (Proteintech 29603-1-AP, 1:1000), anti- β-catenin (Proteintech 51067-2-AP, 1:20 000), anti- βactin (Proteintech 20536-1-AP, 1:10 000), anti- α-tubulin (HuaBio HA721913, 1:5000), anti- β-tubulin (HuaBio ET1602-4, 1:10 000), anti-FLAG (Proteintech 66008-4-Ig, 1:10 000) in TBST for 1.5 h at room temperature. .. After washing three times with TBST, membranes were incubated with secondary antibodies, goat antimouse (Proteintech, RGAM001) or goat anti-rabbit (Proteintech, RGAR001), diluted 1:10 000 in TBST for 1 h at room temperature.

    Western Blot:

    Article Title: SOD1 lactylation impair its enzymatic activity by conformational change to aggravate intervertebral disc degeneration.
    Article Snippet: Proteins were resuspended in SDS– PAGE loading buffer (P0015, Beyotime), boiled, and separated by 10% or 12% SDS–PAGE, then transferred to PVDF membranes (Merck, Germany). .. Membranes were blocked with QuickBlock Western solution (P0252, Beyotime) and incubated overnight at 4 °C with primary antibodies, including anti-P21 (ab109199, Abcam), anti-GADD45α (ab54210, Abcam), anti-SOD1 (ab13498, Abcam), anti-pan lactylation (PTM-1401RM, PTM), anti-SOD1K123la (customized, PTM), anti-pan acetylation (PTM-101, PTM), anti-pan succinylation (PTM-419, PTM), and anti-βactin (66009-1-Ig, Proteintech) antibodies. .. After being washed with TBST, the membranes were incubated with secondary antibodies (SA00001-2 or SA00001-1, Proteintech).

    Article Title: Endogenous Ceramide 24:1 Constrains Th17-Driven Neutrophilic Inflammation by Antagonizing EP2 Signaling.
    Article Snippet: Advanced Science, 2026 om m ons L icense nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.202520695 by IN A SP - N E PA L , W iley O nline L ibrary on [15/03/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C For WB analysis, the protein lysates were separated via SDS ‒PAGE, transferred to PVDF membranes, and incubated overnight at 4◦C with the following primary antibodies: antiSmpd1 (1:1000, ABclonal, #39147-1), anti-Cers2 (1:1000, Abcam, #ab31545), anti-Sptlc2 (1:1000, Abcam, #ab229330), anti-Ror γt (1:1000, Proteintech, #29910-1-AP), anti-Jak2 (1:1000, CST, #3230), anti-p-Jak2 (1:1000, CST, #8082S), anti-Stat3 (1:1000, CST, #4904T), anti-p-Stat3 (1:1000, CST, #9145T), anti-EP2 (1:1000, Abcam, #ab167171), anti-EP4 (1:1000, Proteintech, #24895-1-AP), anti-CXCR4 (1:1000, Abcam, #ab181020), anti-CREB (1:1000, CST, #9197), anti-p-CREB (1:1000, CST, #9198), and anti- βActin (1:1000, Proteintech, #20536-1-AP). ..



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